Creation of transgenic Brassica napus L. Plants expressing human alpha 2b interferon gene
نویسندگان
چکیده
منابع مشابه
Assessment of salt tolerance in transgenic tobacco (Nicotiana tobacum L.) plants expressing the AUX gene
Transformation of plants using Agrabacterium rhizogenes may affect secondary metabolite production as well as morphological changes. In this study, T-DNA from Ri plasmid in A. rhizogenes carrying pRi15834-PRT35S-GUS was introduced into tobacco leaf segments to initiate development of transformed hairy roots. Plant regeneration from transgenic roots used MS medium, and plants regenerated fro...
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A method for transformation of B.napus is presented in this study. The protocol is based on infection of cotyledonary petioles of 5-day-old and hypocotyl segments of 14-day-old seedlings with an Agrobacterium tumefaciens strain LBA 4404 carrying a disarmed binary vector pBI121. The T-DNA region of this binary vector contains the neomycin phosphotransferase II (NPTII) and glucuronidase (GUS) gen...
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This research aimed to find out the impact of ionizing radiations on the hIFNα-2b gene of radiotherapy treated cancer patients. The gene hIFNα-2b synthesizes a protein which is an important anticancerous and antiviral protein. The cancer patients (breast, lung, thyroid, oral and prostate) who were undergoing a radiotherapy treatment were selected. A molecular analysis was performed for DNA isol...
متن کاملassessment of salt tolerance in transgenic tobacco (nicotiana tobacum l.) plants expressing the aux gene
transformation of plants using agrabacterium rhizogenes may affect secondary metabolite production as well as morphological changes. in this study, t-dna from ri plasmid in a. rhizogenes carrying pri15834-prt35s-gus was introduced into tobacco leaf segments to initiate development of transformed hairy roots. plant regeneration from transgenic roots used ms medium, and plants regenerated fro...
متن کاملAltered Fruit and Seed Development of Transgenic Rapeseed (Brassica napus) Over-Expressing MicroRNA394
Fruit and seed development in plants is a complex biological process mainly involved in input and biosynthesis of many storage compounds such as proteins and oils. Although the basic biochemical pathways for production of the storage metabolites in plants are well characterized, their regulatory mechanisms are not fully understood. In this study, we functionally identified rapeseed (Brassica na...
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ژورنال
عنوان ژورنال: Cytology and Genetics
سال: 2012
ISSN: 0095-4527,1934-9440
DOI: 10.3103/s0095452712060096